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ARBOR ASSAYS 免疫測(cè)試產(chǎn)品 試劑盒-相關(guān)技術(shù)文章

更新時(shí)間:2024-07-16      瀏覽次數(shù):174

ARBOR ASSAYS 免疫測(cè)試產(chǎn)品 試劑盒


Arbor Assays公司成立于2007年,位于密歇根州的安娜堡,公司的三位主創(chuàng)始人均為有多年科研經(jīng)驗(yàn)的生命和臨床科學(xué)家。其宗旨是為臨床使用的重要生物分子建立高質(zhì)量的檢測(cè)和免疫測(cè)試產(chǎn)品。在生物領(lǐng)域建立健全的免疫檢測(cè)和酶活性試劑盒以定量生物分子。

主要為臨床重要生物分子提供高質(zhì)量的檢測(cè)和免疫測(cè)試產(chǎn)品,如乙酰膽堿酯酶活性熒光檢測(cè)試劑盒、谷胱甘肽熒光檢測(cè)試劑盒、血紅蛋白比色檢測(cè)試劑盒等。種類多樣,操作簡(jiǎn)單,靈敏度高。

認(rèn)識(shí)到對(duì)靈敏度、速度、便利性、質(zhì)量和服務(wù)的需求,我們研究、開(kāi)發(fā)和制造用于臨床重要生物分子的最高質(zhì)量的檢測(cè)和免疫測(cè)定試劑盒。

Arbor Assays公司的主要產(chǎn)品包括:

DetectX® Kits

Inhibitors & Activators

AbX® Antibodies & Affinity Resins

Detection Systems

Components & Ancillary Reagents

所需材料:

類固醇標(biāo)準(zhǔn)品,用于檢測(cè)萃取效率

ACS級(jí)乙醇(或乙酸乙酯)

玻璃試管

處理流程:

確保樣品wan全干燥,并對(duì)樣品進(jìn)行粉末化處理,以提高萃取回收率。如果可能的話清除任何大顆粒,如草屑。我們建議通過(guò)使用試劑盒的AB分析緩沖液及已知濃度的類固醇溶液檢測(cè)結(jié)果來(lái)確定提取效率,詳細(xì)信息請(qǐng)參考下面的萃取效率部分。乙醇或乙酸乙酯萃取樣品及萃取效率控制如下:

1.稱取≥0.2 g干燥的糞便固體放入管中。樣品可以通過(guò)被動(dòng)干燥、溫和加熱(60°C)或冷凍的方式來(lái)進(jìn)行干燥處理。

2.0.1 g固體(0.1 g糞便固體/mL)加入1 mL乙醇(或乙酸乙酯)并密封。

3.用力搖晃至少30分鐘。

4.4°C下,將樣品在5,000 rpm下離心15分鐘。取上清放入一個(gè)干凈的管中。如果密封得當(dāng),該樣本可在≤-20°C下保存至少一個(gè)月。

注:含有低水平分析物的樣品可以通過(guò)干燥提取液和在少量分析緩沖液中重懸,來(lái)達(dá)到濃縮的效果。用乙酸乙酯提取的樣品在加入緩沖液之前,必須wan全干燥以去除溶劑。從步驟4中轉(zhuǎn)移測(cè)量體積(蒸發(fā)體積)的上清到一個(gè)干凈的管中,在離心蒸發(fā)裝置或氮?dú)庀抡舭l(fā)至干燥。干燥、提取的樣品可在干燥器中冷凍≤-20°C保存。或用乙醇(100μL)溶解干燥提取的樣品,直接進(jìn)行步驟5。

 5.以上步驟為通用步驟,可根據(jù)需要量進(jìn)行等比例體積調(diào)整。將步驟4中提取的樣本上清液100μL加入最少400μLAB緩沖液中(總重構(gòu)體積=100μL+400μL)。室溫下渦旋混勻,靜置5min,重復(fù)至少兩次渦旋和靜置步驟。對(duì)于免疫分析,測(cè)定中乙醇含量通常需要≤5%,需要根據(jù)所選擇的試劑盒中描述的最大乙醇含量耐受情況,用檢測(cè)緩沖液進(jìn)行稀釋。

6.根據(jù)試劑盒說(shuō)明書(shū)對(duì)稀釋后的樣本進(jìn)行ELISA檢測(cè)。

 

下面是ARBOR ASSAYS公司的部分熱銷產(chǎn)品

產(chǎn)品名稱

貨號(hào)

規(guī)格

Goat Anti-Mouse IgG Fc, 25MG

A008-25MG

25MG

Cyclic AMP Direct ELISA Kit (1 Plate)

K019-H1

1x96 well plate

Corticosterone ELISA Kit (1 Plate)

K014-H1

1x96 well plate

Protein Kinase A (PKA) Activity Assay Kit (1 Plate)

K027-H1

1x96 well plate

Aldosterone ELISA Kit (1 Plate)

K052-H1

1x96 well plate

Urea Nitrogen (BUN) Detection Kit (2 Plate)

K024-H1

2x96 well plate

Cortisol ELISA Kit (1 Strip plate)

K003-H1

1x96 well plate

PGFM ELISA Kit (1 Plate)

K022-H1

1x96 well plate

Triiodothyronine (T3) ELISA Kit (1 Plate)

K056-H1

1 Kit

FRAP™ (Ferric Reducing Antioxidant Power) Detection   Kit (2 Plate)

K043-H1

2x96 well plate

Hemoglobin Detection Reagent, 20ML

C038-20ML

20ML

Ceruloplasmin Colorimetric Activity Kit (2 Plate)

K035-H1

2x96 well plate

ThioStar™ Fluorescent Thiol Substrate, 100UG

L002-100UG

100UG

Cyclic GMP Direct Chemiluminescent ELISA Kit (1   Plate)

K020-C1

1x96 well plate

Progesterone ELISA Kit (1 Plate)

K025-H1

1x96 well plate

Testosterone ELISA Kit (1 Plate)

K032-H1

1x96 well plate

Estrone-3-Glucuronide (E1G) ELISA Kit (1 Plate)

K036-H1

1x96 well plate

Thyroxine (T4) ELISA Kit (1 Plate)

K050-H1

1x96 well plate

Prostaglandin E2 (PGE2) Multi-Format ELISA Kit (1   Plate)

K051-H1

1x96 well plate

Tranylcypromine

X042-1EA

1EA

Triiodothyronine (T3) ELISA Kit (5 Plate)

K056-H5

5x96 well plate

Glutathione Colorimetric Detection Kit (4 Plate)

K006-H1

4x96 well plate

Cyclic GMP Direct ELISA Kit (1 Plate)

K020-H1

1x96 well plate

Progesterone ELISA Kit (5 Plate)

K025-H5

5x96 well plate

Cyclic AMP Direct ELISA Kit (5 Plate)

K019-H5

5x96 well plate

Estradiol ELISA Kit (1 Plate)

K030-H1

1x96 well plate

Oxytocin ELISA Kit (1 Plate)

K048-H1

1x96 well plate

Estrone-3-Glucuronide (E1G) ELISA Kit (5 Plate)

K036-H5

5x96 well plate

Pregnanediol-3-Glucuronide (PDG) ELISA Kit (5   Plate)

K037-H5

5x96 well plate

Hemoglobin Standard, 300UL

C037-300UL

300UL

DetectX® Estrone-3-Sulfate (E1S) Antibody, 3ML

C133-3ML

3ML

Creatinine Urinary Detection Kit (2 Plate)

K002-H1

2x96 well plate

Butyrylcholinesterase Fluorescent Activity Kit (2   Plate)

K016-F1

2x96 well plate

Corticosterone ELISA Kit, (5 Plate)

K014-H5

5x96 well plate

Cyclic GMP Direct Chemiluminescent ELISA Kit (5   Plate)

K020-C5

5x96 well plate

Superoxide Dismutase (SOD) Colorimetric Activity   Kit (2 Plate)

K028-H1

2x96 well plate

Catalase Colorimetric Activity Kit (2 Plate)

K033-H1

2x96 well plate

Glutathione Fluorescent Detection Kit (1 Plate)

K006-F1

1x96 well plate

Pregnanediol-3-Glucuronide (PDG) Standard, 625UL

C128-625UL

625UL

Thyroxine (T4) ELISA Kit (5 Plate)

K050-H5

5x96 well plate

Acetylcholinesterase Fluorescent Activity Kit (2   Plate)

K015-F1

2x96 well plate

Creatinine Serum Kit (2 Plate)

KB02-H1

2x96 well plate

Hydrogen Peroxide Fluorescent Detection Kit (2   Plate)

K034-F1

2x96 well plate

Creatinine Serum Low Sample Volume Kit (384-well   Plate)

KB02-H1D

1x384 well plate

Recombinant Obelin, 100UG

L001-100UG

100UG

Dry DMSO, 20ML

X022-20ML

20ML

Dry DMSO, 2ML

X022-2ML

2ML

Urea Nitrogen Standard, 1ML

C089-1ML

1ML

NADPH lyophilized, 1EA

X045-1EA

1EA

DetectX® PGE2 Conjugate, 13ML

C179-13ML

13ML

17-Hydroxyprogesterone   ELISA Kits

K053-H

1 Kit

Arg8-Vasopressin (AVP) ELISA Kits

K049-H

1 Kit

Arg8-Vasopressin (AVP) Chemiluminescent   ELISA Kits

K049-C

1 Kit

Androstenedione ELISA Kits

K070-H

1 Kit

Aldosterone Chemiluminescent ELISA Kits

K052-C

1 Kit

Allopregnanolone ELISA Kits

K061-H

1 Kit

Adrenocorticotropic Hormone (ACTH) ELISA   Kits

K072-H

1 Kit

Alkaline Phosphatase (ALP) Colorimetric   Activity Kit

K082-H1

1 Kit

3,3-Cyclic GAMP ELISA Kits

K073-H

1 Kit

20-Hydroxyecdysone ELISA Kit

K066-H

1 Kit

2,3-Cyclic GAMP STING-Based FRET Detection Kits

K081-F

1 Kit

2,3-Cyclic GAMP ELISA Kits

K067-H

1 Kit

Cyclic GMP Direct ELISA Kits – Improved   Sensitivity

K065-H

1 Kit

Endothelin-1 (ET-1) ELISA Kit

K045-H1

1 Kit

Estrone-3-Sulfate (E1S) ELISA Kits

K038-H

1 Kit

Assay Buffer, 5X

X065-110ml

110ml

Blocking Buffer, 10X

X109-25ml

25ml

Goat anti-Rabbit IgG Coated Plate

X016-1EA


HRP Conjugate Diluent

X076-300ml

300ml

TMB Substrate

X019-55ml

55ml

Mesotocin Solution (500ng/mL)

X127-625UL


5-Azacytidine

P012-50MG

50mg

 

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