PRODUCT CLASSIFICATION
產(chǎn)品分類Cedarlane 淋巴細胞分離柱
Cedarlane公司成立于加拿大,27年間,已經(jīng)逐步發(fā)展成為生命科學(xué)行業(yè)的一家跨國公司,在加拿大和美國擁有超過100名員工。
Cedarlane公司的研究領(lǐng)域。
公司為生命科學(xué)研究人員和臨床醫(yī)生提供無數(shù)的生物和生化產(chǎn)品,提供來自幾乎所有世jie上zui 知 ming 的國際制造商的產(chǎn)品。交貨及時,所有產(chǎn)品都在適當(dāng)?shù)乃铚囟认聝Υ?、接收和運輸。員工與客戶和供應(yīng)商密切合作,提供個性化和全面的體驗,以反映客戶至上的核心價值。通過提供全球超過 600 萬種試劑盒和試劑的門戶,客戶擁有貨運整合的優(yōu)勢以及其中固有的便利性和成本節(jié)約;Cedarlane 是一站式商店。
主要分銷產(chǎn)品線包括:抗體、細胞培養(yǎng)基和過濾、原代細胞和細胞系、蛋白質(zhì)和肽、酶和抑制劑、成像和顯微鏡資源、檢測/ELISA試劑盒、精細化學(xué)品和生化、DNA/RNA分離和純化。
主要制造產(chǎn)品包括:單克隆和多克隆抗體、細胞分離培養(yǎng)基、組織分型補體和高度表征的神經(jīng)元細胞系。
Lympholyte®-H是一種密度梯度分離介質(zhì),專門設(shè)計用于從人外周血和臍帶血中分離淋巴細胞和單核細胞。
注意:使用前充分搖晃混勻,靜止2-3min,待氣泡消失后試用。(室溫條件下使用分離液,分離液密度會隨溫度的變化而發(fā)生改變)
樣本制備可使用無血清培養(yǎng)基,減少樣本粘度,更利于分離。
操作步驟:
1.在含有抗凝血劑的試管中收集人血;
2.用等量培養(yǎng)基1:1稀釋血液;
3.根據(jù)不同的方法進行分離:
①在離心管中加入3 mL Lympholyte®-H;
②使用移液管,小心地將6 mL稀釋的血液鋪在Lympholyte®-H上,在液面上盡可能少地混合(圖A);
③由于Lympholyte®-H的密度比細胞懸液更大,因此將形成一個明顯的界面(圖C)。
方法B:
①在離心管中加入6 mL稀釋后的血液。將一個大的(23厘米)移液管放置到試管的底部(圖B);
②慢慢地向移液管中加入Lympholyte®-H,重力使其在稀釋的血液下分層;
③繼續(xù)處理,直到3mL Lympholyte®-H在稀釋的血液下分層。由于Lympholyte®-H比細胞懸液密度更大,因此會形成明顯的界面(圖C);
4.在室溫下,以800g離心20分鐘;
5.離心后,界面上將有一個明顯的淋巴細胞層(圖D)。使用移液管,小心地將細胞吸出,并轉(zhuǎn)移到一個新的離心管中;
6.用培養(yǎng)基稀釋轉(zhuǎn)移的細胞,以降低溶液的密度。800g離心10分鐘,沉淀淋巴細胞,然后棄用上清液;
Note:如果需要去除血小板,可在這一步進行操作:
方法A
A.加入0.5 mL緩沖液和50 uL凝血酶,然后輕輕重懸細胞;
B.用緩沖液補足10mL,并充分混合;
C.非常緩慢地(100g)離心細胞3分鐘;血小板會聚集并沉淀在底部;
D. 取出上清液(包含細胞),放入另一個試管中,留下聚集的血小板。
方法B
A.800 g離心1分鐘,沉淀細胞;
B.倒出上清液,用緩沖液重懸細胞,重復(fù)2次;
C.此時會觀察到,當(dāng)血小板被移除時,產(chǎn)生的上清液會變得更加清晰。
7.在進一步處理之前,在培養(yǎng)基中清洗淋巴細胞2-3次(在這個階段可以使用含有血清的培養(yǎng)基)。
以下是Cedarlane公司的熱銷產(chǎn)品
產(chǎn)品名稱 | 貨號 | 規(guī)格 |
Lympholyte®-H Cell Separation Media (Isolation of lymphocytes from Human peripheral blood), sterile liquid | CL5020 | 500ml |
Baby Rabbit Complement, Tissue Culture Grade - Frozen | CL3441-S | 1ml |
Lympholyte®-H Cell Separation Media (Isolation of lymphocytes from Human peripheral blood), sterile liquid | CL5016 | 6x100mL |
Low-Tox® Guinea Pig Complement, Lyophilized | CL4051 | 5x1ml |
Lympholyte®-M Cell Separation Media (Isolation of lymphocytes from Mouse lymphoid tissue), sterile liquid | CL5031 | 100ml |
Lympholyte®-Mammal Cell Separation Media (Isolation of lymphocytes from Mammalian peripheral blood), sterile liquid | CL5115 | 100ml |
Low-Tox®-M Rabbit Complement, Lyophilized | CL3051 | 5x1ml |
Lympholyte®-M Cell Separation Media (Isolation of lymphocytes from Mouse lymphoid tissue), sterile liquid | CL5030 | 5x30ml |
Lympholyte®-H Cell Separation Media (Isolation of lymphocytes from Human peripheral blood), sterile liquid | CL5015 | 100ml |
Lympholyte®-H Cell Separation Media (Isolation of lymphocytes from Human peripheral blood), sterile liquid | CL5026 | 6x500ml |
Anti-Mouse Asialo GM1, Ig Fraction (Polyclonal)(rabbit Ig) | CL8955 | 1 mL |
Lympholyte®-Mammal Cell Separation Media (Isolation of lymphocytes from Mammalian peripheral blood), sterile liquid | CL5120 | 500ml |
Human Complement, Pooled, Frozen | CL6600-1 | 1 ml |
Anti-Mouse/Human Mac-2 (Galectin-3), Purified (Clone M3/38) (rat IgG2a) | CL8942AP | 100ug |
Anti-Human Tamm Horsfall Purifed (Clone 10.32) (mouse IgG2b) | CL1032AP | 250ug |
Anti-Mouse Complement Component C3, FITC (Clone RmC11H9) (rat IgG2a) | CL7503F | 100ug |
Lympholyte®-Mammal Cell Separation Media (Isolation of lymphocytes from Mammalian peripheral blood), sterile liquid | CL5110 | 5x30ml |
Lympholyte®-1.1, sterile liquid | CL6155 | 500mL |
Baby Rabbit Complement, Lyophilized | CL3441 | 10x1ml |
Anti-Human Factor VIII:C HRPO (Polyclonal) (sheep IgG) | CL20035HP | 0.2 mg |
Anti-Human Factor VIII:C Aff.Pur.HRPO(Paired Abs) | CL20035K | 4 plates |
Low-Tox®-H Rabbit Complement, Lyophilized | CL3331 | 10x1mL |
Chicken Gamma Globulin (purified antigen) | CLF802-1 | 50mg |
Lympholyte®-Rat Cell Separation Media (Isolation of lymphocytes from Rat lymphoid tissue), sterile liquid | CL5041 | 100mL |
Lympholyte®-Rabbit Cell Separation Media (Isolation of lymphocytes from Rabbit lymphoid tissue), sterile liquid | CL5050 | 5x30ml |
Standard Rabbit Complement, Lyophilized | CL3111 | 25x1ml |
Anti-Rat CD90 (Thy 1.1) Ascites (Clone OX-7) (mouse IgG1) | CL005A | 0.5ml |
Rabbit Complement MA, Lyophilized | CL3221 | 10x1ml |
Lympholyte®-poly Cell Separation Media (Isolation of PMNs from Human peripheral blood), sterile liquid | CL5070 | 100mL |
Anti-Human Fibrinogen Aff.Pur./HRPO (Paired Abs) | CL20067K | 5 plates |
Lympholyte®-1.1, sterile liquid | CL5095 | 500mL |
O-sialoglycoprotein endopeptidase, Lyophilized | CLE100 | 1.2mg |
Anti-Mouse Thrombocyte (platelet), Antiserum, (Polyclonal) (rabbit serum) | CLA31440 | 5mL |
Anti-Human Tissue Plasminogen Activator, Affinity Purified (Polyclonal) (sheep IgG) | CL20115AP | 0.5 mg |
Anti-Human Factor IX Aff.Pur./HRPO (Paired Abs) | CL20041K | 5 plates |
Baby Rabbit Complement, Tissue Culture Grade, Frozen | CL3441-S100 | 100ml |
Standard Guinea Pig Complement, Lyophilized | CL5000 | 5x5ml |
Lympholyte®-H Cell Separation Media (Isolation of lymphocytes from Human peripheral blood), sterile liquid | CL5010 | 5x30mL |
Human Interleukin-17A | CL101-17-25UG | 25ug |
Anti-Mouse Collagen Type I, purified IgG | CL50151AP-1 | 0.1ml |
Anti-Mouse CD25 , Purified (Clone PC61.5.3) (rat IgG1) | CL8925AP | 250ug |
Anti-Rat IL-10, Purified, (Polyclonal) (rabbit IgG) | CL9583AP | 50ug |
Lympholyte®-poly Cell Separation Media (Isolation of PMNs from Human peripheral blood), sterile liquid | CL5071 | 250 mL |
Anti-Mouse PMN, Antiserum, (Polyclonal) (rabbit serum) | CLA31140 | 5mL |
Anti-Rat Myeloid Lineage, FITC (Clone OX82) (mouse IgG1) | CL081F | 100 ug |
Anti-Rat Macrophage, Adsorbed Antiserum, (Polyclonal) (rabbit serum) | CLAD51240 | 2mL |
Baby Rabbit Complement- Tissue Culture Grade, Frozen | CL3441-S50 | 50 ml |
Recombinant Human Gastrokine 3 | CLPRO2033-2 | 10ug |
Anti-Rat CD45RA/B FITC (Clone OX-33) (mouse IgG1) | CL033F | 100ug |
Mini Super PAP PEN (tip 1/10" diameter, 3 mL) | MU22 | EA |
Anti-Rat CD11b/c Purified (Clone OX-42) (mouse IgG2a) | CL042AP | 250ug |
Anti-Human Factor H (FITC) (Clone: OX-24) (mouse IgG1) | CL7672F | 100 ug |
Anti-Mouse I-Ad, Ascites (Clone 34-5-3S) (mouse IgG2a) | CL8713A | 0.5 mL |
Anti-Human Antithrombin III Affinity Purified (Polyclonal) (sheep IgG) | CL20015AP | 0.5mg |
Cedarlane/Anti-Rat CD45, 10 nm Gold Conjugate,OD3 (Clone OX-1) (mouse lgG1) | CL009G | 0.5 mL |
Cedarlane/Rat IL-2ELISA kit(precoated) | CL00113K-96 | 96T |
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