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您的位置:首頁  /  技術(shù)文章  /  Exalpha 凋亡增殖、炎癥、免疫學(xué)傳染病學(xué)和細(xì)胞間結(jié)構(gòu)研究-相關(guān)技術(shù)文章

Exalpha 凋亡增殖、炎癥、免疫學(xué)傳染病學(xué)和細(xì)胞間結(jié)構(gòu)研究-相關(guān)技術(shù)文章

更新時(shí)間:2024-07-18      瀏覽次數(shù):121

Exalpha 凋亡增殖、炎癥、免疫學(xué)傳染病學(xué)和細(xì)胞間結(jié)構(gòu)研究

 

Exalpha公司簡(jiǎn)介

Exalpha有限公司于2000年在馬薩諸塞州雪莉(美國(guó))成立。該公司主要通過開發(fā)和生產(chǎn)抗體來支持生命科學(xué)領(lǐng)域的研究人員和科學(xué)家。

Exalpha公司產(chǎn)品領(lǐng)域

Exalpha公司其領(lǐng)域涉及信號(hào)通路、凋亡、增殖、炎癥、免疫學(xué)、傳染病學(xué)和細(xì)胞間結(jié)構(gòu)研究等方面。Exalpha主要產(chǎn)品線包括Proliferation&apoptosis, Enzymes&Proteins, Clinical IHC Markers , GPCRs等多個(gè)產(chǎn)品線,可提供給全球的科研實(shí)驗(yàn)室,制藥企業(yè),生物技術(shù)和診斷公司,醫(yī)院等,目前旗下的代理公司遍布全球。

細(xì)胞增殖是生物體的重要生命特征,是生物體生長(zhǎng)、發(fā)育、繁殖以及遺傳的基礎(chǔ)。監(jiān)測(cè)細(xì)胞群的生長(zhǎng)對(duì)細(xì)胞的狀態(tài)研究很有必要??蒲泄ぷ髡邆兘?jīng)常需要檢測(cè)細(xì)胞增殖情況,比如驗(yàn)證抗腫瘤藥物的藥效時(shí),監(jiān)測(cè)細(xì)胞增殖及存活情況;研究腫瘤侵襲與轉(zhuǎn)移模型時(shí),分析細(xì)胞轉(zhuǎn)移或侵襲的路徑;分析創(chuàng)傷的修復(fù)或組織的重建時(shí),檢測(cè)細(xì)胞生長(zhǎng)情況等等。檢測(cè)細(xì)胞增殖及活性變化則可以很好的反映細(xì)胞的生長(zhǎng)狀況。

細(xì)胞增殖的研究方法

細(xì)胞增殖的研究方法有很多種,根據(jù)檢測(cè)原理不同,主要分為DNA合成檢測(cè)法、代謝活性檢測(cè)法、細(xì)胞增殖標(biāo)志物檢測(cè)法等。本文重點(diǎn)介紹DNA合成檢測(cè)之Brdu檢測(cè)法,傳統(tǒng)DNA合成檢測(cè)方法使用胸腺嘧啶核苷(3H-TdR)摻入DNA,然后通過放射自顯影或閃爍計(jì)數(shù)對(duì)DNA進(jìn)行定量,該方法由于存在放射性物質(zhì)、實(shí)驗(yàn)周期長(zhǎng)、操作繁瑣,且需要昂貴的設(shè)備,目前已被BrdU5-Bromo-2-deoxyuridine,5-溴脫氧尿嘧啶核苷)檢測(cè)方法取代,BrdU作為一種胸苷類似物,可選擇性地?fù)饺朐鲋臣?xì)胞的DNA中,而且可穩(wěn)定存在,并帶到子代細(xì)胞中,細(xì)胞經(jīng)過固定和變性處理后,可用免疫學(xué)方法檢測(cè)DNABrdU的含量,從而判斷細(xì)胞的增殖能力。

Exalpha Biologicals品牌BrdU檢測(cè)試劑盒

因組織細(xì)胞內(nèi)無內(nèi)源性BrdU存在,可利用BrdU相關(guān)抗體來檢測(cè)和定位摻入活躍增殖細(xì)胞新合成DNABrdU,Exalpha Biologicals 品牌BrdU檢測(cè)試劑盒的優(yōu)勢(shì)在于使用細(xì)胞層作為固相,將BrdU試劑摻入微孔板中的細(xì)胞,除了對(duì)細(xì)胞增殖情況進(jìn)行評(píng)估外,還可以從單個(gè)培養(yǎng)物中分析細(xì)胞數(shù)量、形態(tài)和細(xì)胞抗原等信息。同時(shí)Exalpha Biologicals 品牌BrdU檢測(cè)試劑盒可通過試劑盒自帶的固定/變性溶液一步完成細(xì)胞固定、透化和DNA變性等實(shí)驗(yàn)流程,大大縮短了實(shí)驗(yàn)時(shí)間。Exalpha Biologicals 品牌BrdU檢測(cè)試劑盒中包含檢測(cè)細(xì)胞增殖活性所需的所有組分、操作簡(jiǎn)便、無放射性、廣泛的樣本適用性,已得到眾多科研工作者的青睞。

 

Exalpha公司熱銷產(chǎn)品

產(chǎn)品名稱

貨號(hào)

規(guī)格

BrdU Cell Proliferation Assay Kit

X1327K1

200Tests

chx 10 (Visual system homeobox 2) (NT) Polyclonal Antibody

X1180P

250ug

EBNA 3C Polyclonal Antibody

F125P

250ug

SURF1 Polyclonal Antibody

X1562P

100ug

Bnip3L (IN) (nix) (bcl 2 family) Polyclonal Antibody

X1120P

100ug

chx 10 (Visual system homeobox 2) (CT) Polyclonal Antibody

X1179P

250ug

Microphthalmia Transcription Factor (MiTF) Monoclonal Antibody

X1405M

100ug

EBNA 3A Polyclonal Antibody

F115P

250ug

CD5 PE Single-Color Reagent

0054

100Tests

PTP mu Monoclonal Antibody

P100M

100ug

CD117 FITC Single-Color Reagent

1172

100Tests

Kv1.5 Potassium Channel Polyclonal Antibody

X1501P

100ug

N-SMase Activation Associated Factor Antigen Immunoaffinity Purified   Polyclonal

X2354P

100ug

Chicken anti Glutathione S Transferase (GST), conjugated with HRP

AGST-HRP

0.1 mg

CD7 PE Single-Color Reagent

0074

100Tests

Protein Phosphatase 1 alpha Polyclonal Antibody

P105P

100ug

CD2 PE Single-Color Reagent

0024

100Tests

Kv1.6Potassium Channel Polyclonal Antibody

X1502P

100ug

Degenerative Spermatocyte Homolog 2 (DEGS2) Antigen Immunoaffinity   Purified Polyclonal

X2356P

100ug

CD8 PE Single-Color Reagent

0084

100Tests

Protein Phosphatase 1 beta Polyclonal Antibody

P110P

100ug

CD2 FITC - CD7 PE Bi-Test Reagents (FITC/RPE)

0027S

50Tests

Kv2.1Potassium Channel Polyclonal Antibody

X1503p

100ug

Sphingomyelin Phosphodiesterase 3 Antigen Immunoaffinity Purified   Polyclonal

X2359P

100ug

Chicken anti HA (Hemagglutinin) Epitope

AHA

0.1 mg

CD13 PE Single-Color Reagent

0134

100Tests

Protein Phosphatase 1 beta Polyclonal Antibody

P115P

100ug

CD3 PE Single-Color Reagent

0034

100Tests

Potassium Channel (Kv3.1b ) Polyclonal Antibody

X1504P

100ug

Monocarboxylate Transporter 3 (MCT3) Antigen Immunoaffinity Purified   Polyclonal

X2361P

100ug

Protein Phosphatase 1 gamma 1 Polyclonal Antibody

P120P

100ug

CD61 PE Single-Color Reagent

0614

100Tests

Potassium Channel (Kv4.2 ) Polyclonal Antibody

X1505P

100ug

Zinc Finger Protein GLI1 Antigen Immunoaffinity Purified Polyclonal

X2363P

100ug

Chicken anti HA (Hemagglutinin) Epitope, conjugated with FITC

AHA-F

0.1 mg

CD16 PE Single-Color Reagent

0164

100Tests

Protein Phosphatase 1 gamma 1 Polyclonal Antibody

P125P

100ug

BrdU (bromodeoxyuridine) Monoclonal Antibody

X1028

100ug

Calcium Channel (a1B) Polyclonal Antibody

X1507P

100ug

Monocarboxylate Transport 2 Antigen Immunoaffinity Purified Polyclonal

X2368P

100ug

Chicken anti HA (Hemagglutinin) Epitope, conjugated with HRP

AHA-HRP

0.1 mg

CD18 PE Single-Color Reagent

0184

100Tests

Protein Phosphatase 1 gamma 2 Polyclonal Antibody

P130P

100ug

Muc1 Monoclonal Antibody

X2783M

100ug

Voltage-dependent T-type calcium channel subunit alpha-1E Polyclonal   Antibody

X1509P

100ug

G-Protein Coupled Receptor 94 Antigen Immunoaffinity Purified   Polyclonal

X2373P

100ug

CD19 PE Single-Color Reagent

0194

100Tests

Protein Phosphatase 2 A/A Polyclonal Antibody

P140P

100ug

CD95 Monoclonal Antibody

X2785M

100ug

Calcium Channel (a1G) Polyclonal Antibody

X1510P

100ug

 


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