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ImmunoChemistry Technologies 細(xì)胞蛋白酶-相關(guān)技術(shù)文章

更新時(shí)間:2024-07-22      瀏覽次數(shù):160

ImmunoChemistry Technologies  細(xì)胞蛋白酶

 

ImmunoChemistry公司成立于1994年,作為研發(fā)、銷售于一體的免疫化學(xué)生物技術(shù)公司,提供各種du特的細(xì)胞蛋白酶、細(xì)胞凋亡與細(xì)胞毒性檢測(cè)試劑盒。

美國(guó)ImmunoChemistry TechnologiesICT有幾十年免疫檢測(cè)的經(jīng)驗(yàn),主要提供高質(zhì)量高靈敏度的ELISA以及相關(guān)試劑,包括包被液、封閉液、沖洗液、底物、終止液、板子等等。同時(shí)提供全面的細(xì)胞活性檢測(cè)相關(guān)試劑,包括凋亡、caspase、自噬、細(xì)胞毒性、壞死等相關(guān)檢測(cè)。

ImmunoChemistry公司產(chǎn)品FLICA™、Magic Red™MitoPT™、FLISP™、Cholinesterasedan堿酯酶等全細(xì)胞蛋白酶檢測(cè)試劑盒,不采用酶聯(lián)免疫方法也不使用抗體,通過使用熒光標(biāo)記的滲透細(xì)胞底物與由活性蛋白酶標(biāo)記多肽序列的抑制劑,對(duì)細(xì)胞內(nèi)活性凋亡蛋白酶、組織蛋白酶、絲an酸蛋白酶、dan堿酯酶、功能性線粒體進(jìn)行測(cè)定,便可定量分析細(xì)胞凋亡與細(xì)胞毒性。

一旦小鼠單克隆細(xì)胞系被亞克隆為單克隆,它就可以通過體外(生物反應(yīng)器)或體內(nèi)(腹水)生產(chǎn)用作單克隆抗體(mAb)的無限來源。

方法概述:體外生物反應(yīng)器生產(chǎn)

細(xì)胞系擴(kuò)增(1-2周)

解凍并擴(kuò)增單克隆細(xì)胞系用于接種生物反應(yīng)器。

可根據(jù)要求提供細(xì)胞系對(duì)無血清培養(yǎng)基的適應(yīng)(適應(yīng)最多需要 3 周)。

生物反應(yīng)器生產(chǎn)(2周)

將細(xì)胞系接種到生物反應(yīng)器中并產(chǎn)生抗體。

mAb純化

方法概述:體內(nèi)腹水生產(chǎn)

細(xì)胞系擴(kuò)增(項(xiàng)目相關(guān)時(shí)間線)

解凍并擴(kuò)增單克隆細(xì)胞系用于接種小鼠。

接種(3-4周)

對(duì)小鼠進(jìn)行啟動(dòng),接種細(xì)胞,然后收獲抗體。

mAb純化(3周)

 

ImmunoChemistry Technologies公司熱銷產(chǎn)品

產(chǎn)品名稱

貨號(hào)

規(guī)格

Neptune™ Block ELISA Blocking Buffer

ICT-62

100 mL

Rapid Test Mouse Monoclonal Isotyping Kit

ICT-998

10 tests

General Assay Diluent

ICT-620

100 mL

Cathepsin B (Human Liver)

ICT-6202

25 ug

Cathepsin D (Human Liver)

ICT-6203

25 ug

Camptothecin

ICT-6208

25 mg

Camptothecin

ICT-6209

100 mg

General Assay Diluent

ICT-621

500 mL

Camptothecin

ICT-6210

250 mg

Granzyme B (human, recombinant)

ICT-6211

5000 U

Staurosporine

ICT-6212

1 mg

TNF Alpha

ICT-6213

100 ug

TNF Beta

ICT-6214

20 ug

General Assay Diluent

ICT-622

1 L

IgM-Reducing Assay Diluent

ICT-623

100 mL

IgM-Reducing Assay Diluent

ICT-624

500 mL

DAPI blue fluorescent nuclear DNA dye

ICT-6244

10 mg

Antigen Coating Buffer, 5X

ICT-6247

100 mL

Antigen Coating Buffer, 5X

ICT-6248

500 mL

Antigen Coating Buffer, 5X

ICT-6249

1 L

IgM-Reducing Assay Diluent

ICT-625

1 L

Neptune™ Assay Diluent

ICT-626

100 mL

Phosph-Free Blocking Buffer, Synthetic

ICT-6262

100 mL

Phosph-Free Blocking Buffer, Synthetic

ICT-6263

500 mL

Phosph-Free Blocking Buffer, Synthetic

ICT-6264

1 L

Neptune™ Assay Diluent

ICT-627

500 mL

Alkaline Phosphatase Conjugate Stabilizer, 1X

ICT-6270

25 mL

Alkaline Phosphatase Conjugate Stabilizer, 1X

ICT-6271

100 mL

Alkaline Phosphatase Conjugate Stabilizer, 1X

ICT-6272

500 mL

Alkaline Phosphatase Conjugate Stabilizer, 1X

ICT-6273

1 L

TMB Super Sensitive 1-Component HRP Microwell Substrate

ICT-6275

100 mL

TMB 1-Component HRP Microwell Substrate

ICT-6276

100 mL

TMB Slow Kinetic 1-Component HRP Microwell Substrate

ICT-6277

100 mL

pNPP 1-Component AP Microwell Substrate

ICT-6279

100 mL

Neptune™ Assay Diluent

ICT-628

1 L

TMB 1-Component HRP Membrane Substrate

ICT-6280

100 mL

BCIP/NBT 1-Component AP Membrane Substrate

ICT-6281

100 mL

Stop Solution for TMB Substrates (liquid)

ICT-6282

100 mL

Stop Solution for AP Substrates

ICT-6284

100 mL

ELISA Plate Sealing Covers, 4"x6"

ICT-6287

10/pack

Foil Bags for Plate Storage, 5.25"x6.75"

ICT-6288

10/pack

Desiccant Packs, 5gm each

ICT-6289

10/pack

Antigen-Down Assay Diluent

ICT-629

100 mL

96-Well Plate Template Post-It Notes, 4"x6"

ICT-6290

100 sheets

Goat anti-Human IgG Fc, HRP, AffiPure

ICT-6291

1.0 mg

Goat anti-Mouse IgG Fc, HRP, AffiPure

ICT-6292

1.0 mg

Goat anti-Rabbit IgG Fc, HRP, AffiPure

ICT-6293

1.0 mg

Conjugation-Ready HRP Maleimide

ICT-6294

5.0 mg

Monster Block™ ELISA Blocking Buffer, Non-Mammalian

ICT-6295

100 mL

Monster Block™ ELISA Blocking Buffer, Non-Mammalian

ICT-6296

500 mL

 


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